HighYield T7 Biotin16 RNA Labeling Kit (UTP-based)

HighYield T7 Biotin16 RNA Labeling Kit (UTP-based) is designed to produce randomly Biotin-modified RNA probes with a 16-atom linker viain vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The labeling principle is similar to the underlying labeling principles of Biotin RNA Labeling Mix (Roche) . Biotin-16-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% Biotin-16-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Biotin-16-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Biotin-modified RNA probe with a 16-atom linker can subsequently be detected by HRP-or AP-modified streptavidin. The kit contains sufficient reagents for 30 labeling reactions of 20 μl each (35% Biotin-16-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP) .