UDP-6-azide-glucose
UDP-6-azide-glucose
Product Specifications
Product Name Alternative
Sodium salt, UDP-6-N3-Glc, 6-N3-UDPG
Field of Research
Pharmacology & Drug Discovery
Purity
98% (HPLC)
Form
Solid; Color: white to off-white
Solubility
Water, aqueous buffer such as PBS
Smiles
[C@H]1 ([C@H] (C (O[C@@H] ([C@H]1O) CN=[N+]=[N-]) OP (=O) (OP (=O) (OC[C@H]1O[C@H] ([C@@H] ([C@@H]1O) O) n1c (=O) [nH]c (=O) cc1) O) O) O) O
Molecular Formula
C15H23N5O16P2
Molecular Weight
Theoretical MW: 591.32 g/mol (free acid) ; Detected MW: 591.06 g/mol (free acid)
Storage Conditions
Store at -20 C
Notes
For research use only.
Applications Notes
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 -Glycosyltransferase (T4 -GT) . The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu (I) -free click chemistry that offers the choice: to introduce a (Desthio) Biotin group for subsequent purification tasks (via DBCO-containing (Desthio) Biotin) ; to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes) . 0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 l of a 3 mM solution.
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