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UDP-6-azide-glucose

UDP-6-azide-glucose

Product Specifications

Product Name Alternative

Sodium salt, UDP-6-N3-Glc, 6-N3-UDPG

Field of Research

Pharmacology & Drug Discovery

Purity

98% (HPLC)

Form

Solid; Color: white to off-white

Solubility

Water, aqueous buffer such as PBS

Smiles

[C@H]1 ([C@H] (C (O[C@@H] ([C@H]1O) CN=[N+]=[N-]) OP (=O) (OP (=O) (OC[C@H]1O[C@H] ([C@@H] ([C@@H]1O) O) n1c (=O) [nH]c (=O) cc1) O) O) O) O

Molecular Formula

C15H23N5O16P2

Molecular Weight

Theoretical MW: 591.32 g/mol (free acid) ; Detected MW: 591.06 g/mol (free acid)

Storage Conditions

Store at -20 C

Notes

For research use only.

Applications Notes

Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 -Glycosyltransferase (T4 -GT) . The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu (I) -free click chemistry that offers the choice: to introduce a (Desthio) Biotin group for subsequent purification tasks (via DBCO-containing (Desthio) Biotin) ; to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes) . 0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 l of a 3 mM solution.

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