CuAAC Reaction Ligand Test Kit (THPTA & BTTAA based)

The CuAAC Reaction Ligand Test Kit is suitable to perform Copper (Cu (I) ) -catalyzed Azide-Alkyne Click chemistry reactions (CuAAC) with Azide-or Alkyne-modified biomolecules and cells containing metabolically functionalized Alkyne-or Azide-modified biomolecules. It allows a direct comparison of BTTAA and THPTA to find the most suitable Cu (I) -stabilizing ligand for your experimental set-up. 1 Kit provides sufficient amounts to perform 25 CuAAC experi-ments each with THPTA and BTTAA 200 l using 2 mM CuSO 4 (copper source), 10 mM THPTA or 10 mM BTTAA (Cu (I) -stabilizing ligands) and 100 mM Na-Ascorbate (reduction reagent) in 100 mM Na-Phosphate reaction buffer.