DEAC-dUTP

DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/70-50% dTTP.

Product Specifications

Product Name Alternative

Aminoallyl-dUTP-DEAC, Diethylaminocoumarin-5-dUTP, Diethylaminocoumarin-5-aminoallyl-2-deoxy-uridine-5-triphosphate, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: yellow; pH: 7.5 ± 0.5

Smiles

N (C/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) C (=O) c1cc2c (oc1=O) cc (cc2) N (CC) CC

Molecular Formula

C26H33N4O17P3

Molecular Weight

Theoretical MW: 766.48 g/mol (free acid) ; Detected MW: 766.11 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 426 nm, λem 480 nm, ε 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .