AMCA-6-dUTP
AMCA-6-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended AMCA-6-dUTP/dTTP ratio for PCR and Nick Translation: 20-30% AMCA-6-dUTP/80-70% dTTP (PCR), 30-50% AMCA-6-dUTP/70-50% dTTP (Nick Translation) .
Product Specifications
Product Name Alternative
Aminoallyl-dUTP-AMCA, Aminomethylcoumarin-6-dUTP, Aminomethylcoumarin-5-aminoallyl-2-deoxy-uridine-5-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C30H40N5O18P3
Molecular Weight
Theoretical MW: 851.58 g/mol (free acid) ; Detected MW: 851.16 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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