PCp-Biotin
PCp-Biotin enables 3'-end labeling of RNA/miRNA using T4 RNA Ligase. These probes support hybridization experiments like microarrays or EMSA. Detection utilizes streptavidin-conjugated HRP, AP, or dyes. Alternatively, streptavidin beads can purify labeled probes for further analysis.
Product Specifications
Product Name Alternative
Cytidine-5-phosphate-3- (6-aminohexyl) phosphate, labeled with Biotin, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Liquid
Smiles
Nc1nc (=O) n (cc1) [C@@H]1O[C@H] (COP (=O) (O) O) [C@H] ([C@H]1O) OP (=O) (OCCCCCCNC (=O) CCCC[C@@H]1SC[C@H]2[C@@H]1NC (=O) N2) O
Molecular Formula
C25H42N6O13P2S1
Molecular Weight
Theoretical MW: 728.65 g/mol (free acid) ; Detected MW: 728.20 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. Spectroscopic Propertie: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
Preservative
10 mM Tris-HCl
Frequently Asked Questions
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