DIG-11-ddUTP
Digoxigenin-11-ddUTP is enzymatically incorporated into the 3'-End of oligonucleotides. It acts as a chain terminator leading to the addition of one Digoxigenin label per molecule only. The resulting Digoxigenin-labeled oligonucleotide probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups: 10:1.
Product Specifications
Product Name Alternative
Digoxigenin-3-O-methylcarbonyl-ε-aminocaproyl-[5- (3-aminopropargyl) -2',3'-dideoxyuridine-5'-triphosphate], Triethylammoniumsalt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C43H63N4O20P3
Molecular Weight
Theoretical MW: 1048.91 g/mol (free acid) ; Detected MW: 1048.32 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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