PCp-Cy5

PCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA) .

Product Specifications

Product Name Alternative

Cytidine-5'-phosphate-3'- (6-aminohexyl) phosphate, labeled with Cy5, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Solution in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5

Smiles

C (=C\C1=* (CC) c2c (C1 (C) C) cc (S (=O) (=O) [O-]) cc2) /C=C/C=C\1/N (c2c (C1 (C) C) cc (cc2) S (=O) (=O) O) CCCCCC (=O) NCCCCCCOP (=O) (O[C@@H]1[C@@H] (COP (=O) (O) O) O[C@@H] (n2c (=O) nc (N) cc2) [C@@H]1O) O

Molecular Formula

C48H66N6O18P2S2

Molecular Weight

Theoretical MW: 1141.14 g/mol (free acid) ; Detected MW: 1140.34 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .