Aminoallyl-dUTP-5/6-TAMRA
Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/65% dTTP.
Product Specifications
Product Name Alternative
Tetramethyl-Rhodamine-5-dUTP, 5- (3-Aminoallyl) -2'-deoxyuridine-5'triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: pink to red; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C37H40N5O18P3
Molecular Weight
Theoretical MW: 935.66 g/mol (free acid) ; Detected MW: 935.16 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF740 conjugate, 0.1mg/mL
CD31 / PECAM-1 (158-2B3), CF594 conjugate, 0.1mg/mL
Ep-CAM / CD326 (Epithelial Marker) (rVU-1D9), CF647 conjugate, 0.1mg/mL
MUC18 / Mucin 18 / CD146 (OJ79c), CF740 conjugate, 0.1mg/mL
Cytokeratin 8/18 (C-51), CF405S conjugate, 0.1mg/mL
