2-Ethynyl-ATP (2-EATP)
2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitropolyadenylation of RNA with recombinant poly (A) polymerase. The resulting Alkyne-functionalized RNA can subsequently be processed via Cu (I) -catalyzed Azide-Alkyne click chemistry (CUAAC) that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin) ; to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes) ; to crosslink the RNA to azide-functionalized biomolecules e.g.proteins.
Product Specifications
Product Name Alternative
2-Ethynyl-adenosine-5’-triphosphate, Sodium salt
Concentration
10 mM-11 mM
Purity
90% (HPLC), contains approx. 6% 2-Ethynyl-ADP
Form
Solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 0.5
Smiles
[C@@H]1 ([C@@H] (O[C@@H] ([C@H]1O) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1cnc2c (nc (nc12) C#C) N) O
Molecular Formula
C12H16N5O13P3
Molecular Weight
Theoretical MW: 531.20 g/mol; Detected MW: 531.00 g/mol
Storage Conditions
Store at -20 C
Notes
For research use only.
Applications Notes
In vitro polyadenylation of RNA. Spectroscopic Propertie: max 265 nm, 10.6 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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