Biotin-14-dATP

Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.

Product Specifications

Product Name Alternative

Biotin-14-N6- (6-Aminohexyl) -dATP, Triethylammonium salt, Bio-14-dATP

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@@H] (n2cnc3c (NCCCCCCNC (=O) CCCCCNC (=O) CCCC[C@@H]4SC[C@@H]5NC (=O) N[C@H]45) ncnc23) C[C@@H]1O

Molecular Formula

C32H54N9O15P3S

Molecular Weight

Theoretical MW: 929.81 g/mol (free acid) ; Detected MW: 929.27 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .