HighYield T7 Digoxigenin RNA Labeling Kit (UTP-based)

HighYield T7 Digoxigenin RNA Labeling Kit (UTP-based) is designed to produce randomly Digoxigenin-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The labeling principle is similar to the underlying labeling principles of DIG RNA Labeling Mix (Roche) and DIG RNA Labeling Kit (Roche) . DIG-11-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% DIG-11-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of DIG-11-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Digoxigenin-modified RNA probe can subsequently be detected by HRP-or AP-modified Digoxigenin antibody. The kit contains sufficient reagents for 30 labeling reactions of 20 μl each (35% DIG-11-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM DIG-11-UTP) .