GFP11

GFP11 is a protein fusion tag composed of 16 amino acids. It facilitates the presentation of proteins on the outer membrane of Gram-negative bacteria and emits green fluorescence by complementing with externally added GFP1-10. Due to its small molecular weight, GFP11 does not significantly affect protein solubility or expression, nor does it alter the catalytic activity of the enzyme CsBglA. GFP11 is applicable in creating a split GFP system for high-throughput biotechnology and flow cytometry applications.