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Recombinant Human Interleukin-21 (IL21) (Active)

Recombinant Human Interleukin-21 (IL21) (Active)

Product Specifications

Product Name Alternative

Interleukin-21; IL-21; Za11

UniProt

Q9HBE4

Expression Region

23-155aa

Tag

C-terminal hFc1-tagged

Source

Mammalian cell

Origin

Homo sapiens (Human)

Field of Research

Immunology & Inflammation

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 90% as determined by SDS-PAGE.

Bioactivity

1Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL21R at 2 μg/mL can bind Human IL21. The EC50 is 15.01-19.43 ng/mL. 2Measured by its binding ability in a functional ELISA. Immobilized Human IL21R at 2 μg/mL can bind Human IL21. The EC50 is 16.65-19.49 ng/mL. 3Human IL21 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL21R with an affinity constant of 1.31 nM as detected by MetaSPR Assay. 4Human IL21 captured on Protein A Chip can bind Recombinant Human IL21R with an affinity constant of 1.87 nM as detected by MetaSPR Assay.

Form

Lyophilized powder

Molecular Weight

44.4 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

AA Sequence

QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

Available Sizes

Frequently Asked Questions

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