Recombinant Human Interleukin-21 (IL21) (Active)
Recombinant Human Interleukin-21 (IL21) (Active)
Product Specifications
Product Name Alternative
Interleukin-21; IL-21; Za11
UniProt
Q9HBE4
Expression Region
23-155aa
Tag
C-terminal hFc1-tagged
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 90% as determined by SDS-PAGE.
Bioactivity
1Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL21R at 2 μg/mL can bind Human IL21. The EC50 is 15.01-19.43 ng/mL. 2Measured by its binding ability in a functional ELISA. Immobilized Human IL21R at 2 μg/mL can bind Human IL21. The EC50 is 16.65-19.49 ng/mL. 3Human IL21 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL21R with an affinity constant of 1.31 nM as detected by MetaSPR Assay. 4Human IL21 captured on Protein A Chip can bind Recombinant Human IL21R with an affinity constant of 1.87 nM as detected by MetaSPR Assay.
Form
Lyophilized powder
Molecular Weight
44.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS
Available Sizes
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