Beta-2-microglobulin Antibody
Goat polyclonal to B2M.
Product Specifications
Product Name Alternative
Anti B2M antibody
Reactivity
Human
Target
Beta-2-microglobulin
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QRTPKIQVYSRHP
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary testing showed a band at approx. 12-13kDa in Human Spleen lysate and in Jurkat, U251, U2OS, U937 and HeLa cell lysate after 0.3-1μg/ml antibody staining (calculated MW of 13.7kDa according to NP_004039.1) . Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the plasma membranes and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Molecular Weight
13.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Prediction Reactivity
Bovine
Tested Applications
ELISA, FC, IF
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
567
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