Recombinant COVID-19 S-trimer Protein (BF.7)
Recombinant COVID-19 S-trimer Protein is produced by the mammalian expression system and the target gene encoding Cys15-Gln1208 is expressed with a 6His tag at the C-terminus. T19I, LPP24-26del, A27S, HV69-70del, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K mutations were identified in the COVID-19 variant (known as BF.7 lineage) which emerged in Europe. Six proline substitutions and furin cleavage site substitutions are introduced to stabilize the trimeric prefusion state of COVID-19 S protein and abolish the furin cleavage site, respectively.
Product Specifications
Product Name Alternative
SARS-CoV-2 S protein; SARS-CoV-2 Spike glycoprotein; SARS-CoV-2 S glycoprotein; SARS-CoV-2 S-trimer Protein
UniProt
P0DTC2
Reactivity
Virus
Source
Human cells
Field of Research
Infectious Disease & Virology
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Please minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Entrez
43740568
Available Sizes
Frequently Asked Questions
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