What is the catalog number for PCNA Antibody?

The catalog number for PCNA Antibody is orb12280.

What sizes are available for PCNA Antibody?

PCNA Antibody is available in the following size(s): 100 µg.

Is PCNA Antibody in stock?

PCNA Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PCNA Antibody

Goat polyclonal to PCNA.

Product Specifications

Product Name Alternative

Anti PCNA antibody

Reactivity

Human, Mouse, Porcine, Rat

Target

PCNA

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NGNIKLSQTSNVD

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.01-0.03 μg/ml

Molecular Weight

28.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1) . Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa acco. IHC: Paraffin embedded Human Testis. Recommended concentration: 5-6μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.