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VEGFA (aa288-299) Antibody

Goat polyclonal antibody to VEGF

Product Specifications

Product Name Alternative

Anti Vascular endothelial growth factor A antibody, anti Vascular Endothelial Growth Factor antibody, anti Vascular Permeability Factor antibody, anti VEGF-A antibody, anti VEGFA antibody, anti VPF antibody

Reactivity

Human

Target

VEGFA (aa288-299)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RIKPHQGQHIGE

Field of Research

Cardiovascular Research; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

45.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney. IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5μg/ml.. Notes: Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.

Prediction Reactivity

Canine, Porcine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7422

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