VEGFA (aa288-299) Antibody
Goat polyclonal antibody to VEGF
Product Specifications
Product Name Alternative
Anti Vascular endothelial growth factor A antibody, anti Vascular Endothelial Growth Factor antibody, anti Vascular Permeability Factor antibody, anti VEGF-A antibody, anti VEGFA antibody, anti VPF antibody
Reactivity
Human
Target
VEGFA (aa288-299)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RIKPHQGQHIGE
Field of Research
Cardiovascular Research; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
45.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney. IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5μg/ml.. Notes: Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.
Prediction Reactivity
Canine, Porcine
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
7422
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