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Recombinant Macaca fascicularis Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

Recombinant Macaca fascicularis Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

Product Specifications

Product Name Alternative

Interleukin-2 receptor subunit alpha

UniProt

H6WS54

Expression Region

22-238aa

Tag

C-terminal 10xHis-tagged

Source

Mammalian cell

Origin

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Field of Research

Immunology & Inflammation

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Bioactivity

1Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Macaca fascicularis IL2. The EC50 is 88.60-116.8 ng/mL. 2Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Human IL2. The EC50 is 0.8151-1.087 ng/mL. 3Macaca fascicularis IL2 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay.

Form

Lyophilized powder

Molecular Weight

26.1 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

AA Sequence

ELCDDDPPKITHATFKAVAYKEGTMLNCECKRGFRRIKSGSPYMLCTGNSSHSSWDNQCQCTSSAARNTTKQVTPQPEEQKERKTTEMQSQMQLADQVSLPGHCREPPPWENEATERIYHFVVGQTVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGETEPSQFPGEEEPQASPDGLPESETSRLVTTTDFRIQTEVAATMETFIFTTE

Available Sizes

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