ITK Antibody
Goat polyclonal to ITK
Product Specifications
Product Name Alternative
Anti ITK antibody
Reactivity
Human, Rat
Target
ITK
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KERPEDRPAFSR
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75ug/ml
Molecular Weight
71.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2) . Recommended concentration: 0.3-1μg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen
Prediction Reactivity
Bovine
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
3702
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