CREB1 (aa96-109) Antibody

Goat polyclonal antibody to CREB1

Product Specifications

Product Name Alternative

Anti CREB1 antibody, anti cAMP responsive element binding protein 1 antibody, anti cAMP-response element-binding protein-1 antibody, anti cAMP-responsive element-binding protein 1 antibody, anti cyclic AMP-responsive element-binding protein 1 antibody, anti transactivator protein antibody

Reactivity

Human, Mouse

Target

CREB1 (aa96-109)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DSQESVDSVTDSQK

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.1-0.3 μg/ml.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

35.1; 36.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3μg/ml. Primary incubation wa. IHC: Paraffin embedded Human Prostate. Recommended concentration: 2.5μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.