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ZNF697 (aa145-157) Antibody

Goat polyclonal to ZNF697

Product Specifications

Product Name Alternative

ZNF697; zinc finger protein 697; MGC45731; OTTHUMP00000015145

Target

ZNF697 (aa145-157)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RHLSLGSRHRGDK

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3 μg/ml

Molecular Weight

60.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Preliminary experiments gave bands at approx 75kDa and 50kDa in HeLa nuclear lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 60.5kDa according to NP_001073939.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Preliminary experiments gave bands at approx 75kDa and 50kDa in HeLa nuclear lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 60.5kDa according to NP_001073939.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

90874

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