Human FGF-8a Protein, His tag (Animal-Free)

Fibroblast Growth Factors-8a (FGF-8a) is a 23.5 kDa member of the fibroblast Growth Factors with 204 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation.

Product Specifications

Background

FGF-8 is a heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF8; FGF-8A, FGF-8B, FGF-8E and FGF-8F. The human and murine FGF-8A and B are identical, unlike human and mouse FGF-8E and F, which are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors.

Product Name Alternative

Fibroblast growth factor 8a, AIGF, HBGF-8, FGF8

Gene ID

2253

Accession Number

P55075

Tag

FGF-8a

Type

Cytokines and proteins

Applications

His tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 μg/mL.

Molecular Formula

22.14 kDa

Molecular Weight

22.14 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin :< 0.1 EU per 1 μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human FGF-8a consists of 182 amino acids and predicts a molecular mass of 22.14 kDa.

Formulation

Lyophilized from sterile PBS, pH 8.0.

Applications Notes

Human

Other Product Names

FGF-8a, FGF8a, FGF 8a, fibroblast growth factor 8a, AIGF, HBGF-8, FGF8

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.