Anti-Fen-1 Antibody

Mouse Monoclonal Antibody specific to Fen-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

FEN-1, EC 3.1.-.-, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

Gene Name

FEN1

NCBI Gene ID

UniProt

P39748

Cellular Locus

[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.

Target Antigen

Flap endonuclease 1

Target

Fen-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {HAMAP-Rule:MF_03140, PubMed:10744741, PubMed:11986308, PubMed:18443037, PubMed:20729856, PubMed:26751069, PubMed:7961795, PubMed:8621570}.

Additionnal Information

Immunoblotting: use at 1-5 ug/mL. <br><br>Positive controls: MCF-7 cells and recombinant fusion protein.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human FEN-1.

Formulation

10 mM PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

FEN-1 (Flap Endonuclease and Five Prime Exonuclease) is a 42 kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 appears to have important roles in DNA replication and repair and is required in cell-fre DNA replication systems.