ATF6 (aa437-449) Antibody
Goat polyclonal antibody to ATF6
Product Specifications
Product Name Alternative
Anti-Activating transcription factor 6 alpha antibody, anti-Activating transcription factor 6 antibody, anti-ATF 6 antibody, anti-ATF6 alpha antibody, anti-ATF6 antibody, anti-ATF6-alpha antibody, anti-ATF6A antibody, anti-cAMP dependent transcription factor ATF 6 alpha antibody, anti-ESTM49 antibody, anti-FLJ21663 antibody, anti-Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
Reactivity
Human
Target
ATF6 (aa437-449)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NSYRYDHSVSNDK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.3-2 μg/ml, IHC-P: 10ug/ml
Molecular Weight
74.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 75-80kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6kDa according to NP_031374.2) . Recommended concentration: 0.3-2μg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
Prediction Reactivity
Bovine, Canine, Porcine, Rat
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
22926
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