Recombinant Human CD40 ligand (CD40LG), partial (Active)

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973) . Costimulates T-cell proliferation and cytokine production (PubMed:8617933) . Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933) . Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973) . Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037) . Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037) . Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity) . Involved in immunoglobulin class switching

①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody (CSB-RA156386A0HU), the EC50 is 2.353-3.232 ng/ml.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.