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PRKCB Antibody

Goat polyclonal to PRKCB

Product Specifications

Product Name Alternative

Anti PRKCB antibody

Reactivity

Human, Rat

Target

PRKCB

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DKEFTRQPVELT

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 1-2μg/ml

Molecular Weight

76.9; 77.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1) . Recommended concentration: 0.1-0.3μg/ml.. IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5579

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