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PTCH1 (aa266-280) Antibody

Goat polyclonal antibody to PTCH1

Product Specifications

Product Name Alternative

Anti PTCH1 antibody, anti patched 1 antibody, anti BCNS antibody, anti FLJ26746 antibody, anti FLJ42602 antibody, anti HPE7 antibody, anti NBCCS antibody, anti PTC antibody, anti PTC1 antibody, anti PTCH11 antibody, anti OTTHUMP00000021709 antibody, anti OTTHUMP00000021710 antibody, anti PTCH protein +12b antibody, anti PTCH protein +4 antibody, anti PTCH protein-10 antibody, anti patched homolog 1 antibody, anti protein patched homolog 1 antibody

Reactivity

Human, Mouse

Target

PTCH1 (aa266-280)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EELKKINYQVDSWEE

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

161; 161; 154; 144

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 1-3μg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex) . Recommended concentration: 8μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Prediction Reactivity

Bovine, Canine, Rat

Tested Applications

ELISA, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5727

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