PTCH1 (aa266-280) Antibody
Goat polyclonal antibody to PTCH1
Product Specifications
Product Name Alternative
Anti PTCH1 antibody, anti patched 1 antibody, anti BCNS antibody, anti FLJ26746 antibody, anti FLJ42602 antibody, anti HPE7 antibody, anti NBCCS antibody, anti PTC antibody, anti PTC1 antibody, anti PTCH11 antibody, anti OTTHUMP00000021709 antibody, anti OTTHUMP00000021710 antibody, anti PTCH protein +12b antibody, anti PTCH protein +4 antibody, anti PTCH protein-10 antibody, anti patched homolog 1 antibody, anti protein patched homolog 1 antibody
Reactivity
Human, Mouse
Target
PTCH1 (aa266-280)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EELKKINYQVDSWEE
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
161; 161; 154; 144
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 1-3μg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex) . Recommended concentration: 8μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5727
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