Recombinant Human Laminin 221 E8 Protein
Recombinant Human Laminin 221 is produced by our Mammalian expression system and the target gene encoding Leu1900-Ala2722&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a 6His, Flag and HA tag at N terminus respectively.
Product Specifications
Product Name Alternative
Laminin 221; LN221
UniProt
P55268, P11047, P24043
Expression Region
Leu1900-Ala2722&Leu1573-Gln1798&Asn1364-Pro1609
Tag
N-His &N-Flag&N-HA
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Human Integrin alpha7X2B beta 1 at 5μg/ml (100 μl/well) can bind Recombinant Human Laminin 221 E8 Protein (Cat#orb3002523) *.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human Laminin 221 E8 Protein (Cat#orb3002523) is 6.06 ng/ml. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 91.8&25.8&28.5 KDa. Observed: (27-31) & (35-45) & (110-145)
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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