Recombinant Human Laminin 521 Protein
Recombinant Human Laminin521 is produced by our Mammalian expression system and the target gene encoding Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a His, Flag and HA tag at N terminus respectively.
Product Specifications
Product Name Alternative
Laminin 521; LN521
UniProt
P55268, P11047, O15230
Expression Region
Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609
Tag
N-His & N-Flag & N-HA
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His) (Cat#orb2992902) at 10μg/ml (100 μl/well) can bind Recombinant Human Laminin521 E8 Protein (Cat#orb3002520) .The ED50 of Recombinant Human Laminin521 E8 Protein (Cat#orb3002520) is 31.75 ng/ml (Regularly tested) .. Cell Based Assay: Measured by its ability to support iPS cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 94 ng/mL. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 86.7&25.8&28.5 KDa. Observed: (28-32) & (38-48) & (100-120) KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB,2% Sucrose,4% Mannitol,0.02%Tween-80, pH 7.4.
Available Sizes
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