Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Recombinant Human Laminin 521 Protein

Recombinant Human Laminin521 is produced by our Mammalian expression system and the target gene encoding Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a His, Flag and HA tag at N terminus respectively.

Product Specifications

Product Name Alternative

Laminin 521; LN521

UniProt

P55268, P11047, O15230

Expression Region

Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609

Tag

N-His & N-Flag & N-HA

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His) (Cat#orb2992902) at 10μg/ml (100 μl/well) can bind Recombinant Human Laminin521 E8 Protein (Cat#orb3002520) .The ED50 of Recombinant Human Laminin521 E8 Protein (Cat#orb3002520) is 31.75 ng/ml (Regularly tested) .. Cell Based Assay: Measured by its ability to support iPS cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 94 ng/mL. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 86.7&25.8&28.5 KDa. Observed: (28-32) & (38-48) & (100-120) KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB,2% Sucrose,4% Mannitol,0.02%Tween-80, pH 7.4.

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

GNAS Protein Lysate (Mouse) with C-HA Tag
22304034 100 µg

GNAS Protein Lysate (Mouse) with C-HA Tag

Sign In for Pricing
View Details
Urine DNA extraction kit
BPC1163 50 Tests

Urine DNA extraction kit

Sign In for Pricing
View Details
CLEC9A, ID (CLEC9A, C-type lectin domain family 9 member A) (MaxLight 650)
MBS6286742-01 0.1 mL

CLEC9A, ID (CLEC9A, C-type lectin domain family 9 member A) (MaxLight 650)

Sign In for Pricing
View Details
CLEC9A, ID (CLEC9A, C-type lectin domain family 9 member A) (MaxLight 650)
MBS6286742-02 5x 0.1 mL

CLEC9A, ID (CLEC9A, C-type lectin domain family 9 member A) (MaxLight 650)

Sign In for Pricing
View Details
PTPN6 Polyclonal Antibody
A71600-050 50 µL

PTPN6 Polyclonal Antibody

Sign In for Pricing
View Details
Spsb3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6484005 3x 1.0 µg

Spsb3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

Sign In for Pricing
View Details