Recombinant Human FGFb Protein

Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Gly132-Ser288 is expressed.

Product Specifications

Product Name Alternative

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

UniProt

P09038

Expression Region

Gly132-Ser288

Tag

Tag free

Field of Research

Cardiovascular Research

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Bioactivity

BLI: Loaded Human FGFb (157AA) (Cat#orb3002481) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.7 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.42 ng/ml (Regularly tested) .

Form

Lyophilized

Molecular Weight

Predicted: 17.4 KDa. Observed: 16 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.