Recombinant Human/Mouse/Rat Activin A Protein
Recombinant Human Activin A is produced by our Mammalian expression system and the target gene encoding Gly311-Ser426 is expressed.
Product Specifications
Product Name Alternative
Inhibin beta A chain; INHBA; Activin A
UniProt
P08476
Expression Region
Gly311-Ser426
Tag
Tag free
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
Cell Based Assay: Measured by its ability to induce SMAD signaling in 293-Activin A Res cells (Cat#Xorb2994727) . The ED50 for this effect is 1-5 ng/mL. (QC verified) . Human intestinal organoids (ipsc-derived) were cultured with Activin A (Cat#orb3002480), BMP-4 (Cat#orb3002515), EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology.
Form
Lyophilized
Molecular Weight
Predicted: 13 KDa. Observed: 15 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 4mM HCl,4%Mannitol.
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