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Recombinant Human IL-7 Protein

Recombinant Human Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Asp26-His177 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Interleukin-7; IL-7; IL7

UniProt

P13232

Expression Region

Asp26-His177

Tag

C-6His

Field of Research

Immunology & Inflammation

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Mouse IL-7RA-Fc (Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7-His (Cat#orb3002479) with an affinity constant of 29.0 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) . The ED50 for this effect is 50-300 pg/ml.

Form

Lyophilized

Molecular Weight

Predicted: 18.4 KDa. Observed: 19-30 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB,150mM NaCl,4%Mannitol, pH 7.4.

Available Sizes

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