Recombinant Human FGF-10 Protein

Cell Based Assay: Measured by its ability to induce FGFRIIIb receptor activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 3.82ng/ml (Regularly tested) . Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478) . The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#orb3002493), R-spondin 1 (Cat#orb3002497), FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483) . The organoids showed good morphology. Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 3 (Cat#orb2993001), KGF (Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753) . DAPI (blue), Ki67 (red) and ER (green) . The activity of Recombinant Human FGF-10 Protein (Cat#orb3002478) was compared to Brand R.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.