Recombinant Human FGFb Protein
Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Pro143-Ser288 is expressed.
Product Specifications
Product Name Alternative
Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
UniProt
P09038
Expression Region
Pro143-Ser288
Tag
Tag free
Field of Research
Cardiovascular Research
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
BLI: Loaded Human FGFb (146AA) (Cat#orb3002475) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.6 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-2.0 ng/ml (QC verified) .
Form
Lyophilized
Molecular Weight
Predicted: 16.3 KDa. Observed: 17 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl,100mM NaCl,0.02%Tween 80,4%Mannitol, pH 7.5.
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