Recombinant Human KGF Protein
Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.
Product Specifications
Product Name Alternative
Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
UniProt
P21781
Expression Region
Cys32-Thr194
Tag
Tag free
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human KGF (Cat. No.:orb3002467) at 5 μg/mL (100 μL/well) can bind Human FGFR2 beta IIIb (C-Fc) (Cat. No.:orb2993044) . The EC50 of Human FGFR2 beta IIIb (C-Fc) (Cat. No.:orb2993044) is 83 ng/mL. (Regularly tested) . Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 3 (Cat#orb2993001), KGF (Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753) . DAPI (blue), Ki67 (red) and ER (green) .
Form
Lyophilized
Molecular Weight
Predicted: 18.9 KDa. Observed: 17 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris,1mM EDTA,5% Trehalose, 0.02% Tween 80, pH 8.0.
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