Recombinant Human FGF-4 Protein
Recombinant Human Fibroblast Growth Factor 4 is produced by our E.coli expression system and the target gene encoding Ser54-Leu206 is expressed.
Product Specifications
Product Name Alternative
Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3
UniProt
P08620
Expression Region
Ser54-Leu206
Tag
Tag free
Field of Research
Stem Cell & Developmental Biology
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
Cell Based Assay: Measured in a cell proliferation assay using MCF7 Human breast cancer cells.The ED50 for this effect is 2-20 ng/ml (QC verified) . Human intestinal organoids (ipsc source) were cultured with Activin A (Cat#orb3002480), BMP-4 (Cat#orb3002515), EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology.
Form
Lyophilized
Molecular Weight
Predicted: 16.9 KDa. Observed: 16 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mMPB,5%Sucrose,4%Mannitol,0.02%Tween80, pH7.4.
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