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Recombinant Human TGF-beta 1 Protein (Biotin)

Biotinylated Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed with a Avi tag at the N-terminus.

Product Specifications

Product Name Alternative

Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB

UniProt

P01137

Expression Region

Ala279-Ser390

Tag

N-Avi

Field of Research

Immunology & Inflammation

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

BLI: Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR1 (Cat#orb2992807) with an affinity constant of 0.788 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2 (Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested) . ELISA: Immobilized Biotinylated Human TGF-beta 1-Avi (Cat#orb3002438) at 2μg/ml (100 μl/well) can bind Human TGFBR2-Fc (Cat#orb3002421) .The ED50 of Human TGFBR2-Fc (Cat#orb3002421) is 9.47ng/ml. (Regularly tested) . Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‐1 human erythroleukemic cells.The ED50 for this effect is 137.37pg/ml. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 14.6 KDa. Observed: 12-18 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.

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