Recombinant Human TGF-beta 1 Protein (Biotin)
Biotinylated Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed with a Avi tag at the N-terminus.
Product Specifications
Product Name Alternative
Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
UniProt
P01137
Expression Region
Ala279-Ser390
Tag
N-Avi
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
BLI: Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR1 (Cat#orb2992807) with an affinity constant of 0.788 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2 (Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested) . ELISA: Immobilized Biotinylated Human TGF-beta 1-Avi (Cat#orb3002438) at 2μg/ml (100 μl/well) can bind Human TGFBR2-Fc (Cat#orb3002421) .The ED50 of Human TGFBR2-Fc (Cat#orb3002421) is 9.47ng/ml. (Regularly tested) . Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‐1 human erythroleukemic cells.The ED50 for this effect is 137.37pg/ml. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 14.6 KDa. Observed: 12-18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.
Available Sizes
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