Recombinant Human TGF-beta 1 Protein (Biotin)

BLI: Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR1 (Cat#orb2992807) with an affinity constant of 0.788 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2 (Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested) . ELISA: Immobilized Biotinylated Human TGF-beta 1-Avi (Cat#orb3002438) at 2μg/ml (100 μl/well) can bind Human TGFBR2-Fc (Cat#orb3002421) .The ED50 of Human TGFBR2-Fc (Cat#orb3002421) is 9.47ng/ml. (Regularly tested) . Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‐1 human erythroleukemic cells.The ED50 for this effect is 137.37pg/ml. (Regularly tested)

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.