Recombinant Human IL-4 Protein
Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
UniProt
P05112
Expression Region
His25-Ser153
Tag
C-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
BLI: Loaded Cynomolgus IL-4RA-Fc (Cat#orb2993918) on Protein A Biosensor, can bind Human IL-4-His (Cat#orb3002435) with an affinity constant of 0.33 nM as determined in BLI assay. Loaded Human IL-4RA-Fc (Cat#orb2994196) on Protein A Biosensor, can bind Human IL-4-His (Cat#orb3002435) with an affinity constant of 0.99 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using TF‐1 human erythroleukemic cells.The ED50 for this effect is 0.05-0.5 ng/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 16 KDa. Observed: 17-22&15 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
VAng-Cr3302-1mgEcoli 1 mg (E. coli)
Recombinant Vibrio Cholerae phnX Protein (aa 1-113)
Sign In for Pricing
View Details 
