Recombinant Human IFN alpha2b Protein
Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 is expressed.
Product Specifications
Product Name Alternative
Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2
UniProt
P01563
Expression Region
Cys24-Glu188
Tag
Tag free
Field of Research
Infectious Disease & Virology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Bioactivity
BLI: Loaded Human IFNAR2-Fc (Cat#orb2994011) on Protein A Biosensor, can bind Human IFN alpha2b (Cat#orb3002431) with an affinity constant of 2.98 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL.
Form
Lyophilized
Molecular Weight
Predicted: 19.4 KDa. Observed: 17 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Available Sizes
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