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Recombinant Human NT-3 Protein

Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.

Product Specifications

Product Name Alternative

Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3

UniProt

P20783

Expression Region

Tyr139-Thr257

Tag

Tag free

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Bioactivity

ELISA: Immobilized Human TrkB (C-6His) (Cat. No.:orb2995029) at 5 μg/mL (100 μL/well) can bind Human NT-3 (Cat. No.:orb3002430) *: Biotinylated by NHS-biotin prior to testing. The EC50 of Human NT-3 (Cat. No.:orb3002430) * is 15 ng/mL. (Regularly tested) .

Form

Lyophilized

Molecular Weight

Predicted: 13.6 KDa. Observed: 14 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Available Sizes

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