Recombinant Human NT-3 Protein
Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.
Product Specifications
Product Name Alternative
Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3
UniProt
P20783
Expression Region
Tyr139-Thr257
Tag
Tag free
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Bioactivity
ELISA: Immobilized Human TrkB (C-6His) (Cat. No.:orb2995029) at 5 μg/mL (100 μL/well) can bind Human NT-3 (Cat. No.:orb3002430) *: Biotinylated by NHS-biotin prior to testing. The EC50 of Human NT-3 (Cat. No.:orb3002430) * is 15 ng/mL. (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 13.6 KDa. Observed: 14 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Available Sizes
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