Recombinant Mouse IL-7 Protein
Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF
UniProt
P10168
Expression Region
Glu26-Ile154
Tag
C-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Bioactivity
BLI: Loaded Mouse IL-7RA-Fc (Cat#orb2994406) on Protein A Biosensor, can bind Mouse IL-7-His (Cat#orb3002426) with an affinity constant of 18.9 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) .The ED50 for this effect is 60-1000 pg/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 15.9 KDa. Observed: 22-28 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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