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Recombinant Mouse LIF Protein

Recombinant Mouse Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser24-Phe203 is expressed.

Product Specifications

Product Name Alternative

Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor

UniProt

P09056

Expression Region

Ser24-Phe203

Tag

Tag free

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

Cell Based Assay: Measured by the dose dependent inhibition of M1 cells. The ED50 for this effect is 0.33ng/ml (Regularly tested) . The activity of Recombinant Mouse LIF Protein (Cat#orb3002423) was compared to Brand M.Measured by the dose dependent inhibition of M1 cells.

Form

Lyophilized

Molecular Weight

Predicted: 19.9 KDa. Observed: 18 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Available Sizes

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