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Recombinant Human TGFBR2 Protein

Recombinant Human Transforming Growth Factor-beta Receptor Type II is produced by our Mammalian expression system and the target gene encoding Thr23-Asp159 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

TGF-beta receptor type-2; TGF-beta type II receptor; TGFBR2; Transforming growth factor-beta receptor type II

UniProt

P37173

Expression Region

Thr23-Asp159

Tag

C-Fc

Field of Research

Cell Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

BLI: Loaded Biotinylated Human TGF-beta 1 (Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2 (Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 3 (Cat#orb2993088) on SA Biosensor, can bind Human TGFBR2-Fc (Cat#orb3002421) with an affinity constant of 0.38 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‐1 human erythroleukemic cells. The ED50 for this effect is 18.41 ng/ml in the presence of 100pg/ml of recombinant human TGF-beta 1. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 42.6 KDa. Observed: 59 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Available Sizes

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